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The challenge of measuring the concentrations of morphine and its active metabolites in order to assess human pharmacokinetics and monitor therapeutic drugs in children requires assays with high sensitivity in small blood nightcore everybody loves me female version of viagra. We developed and validated a semi-automated LC-MSMS assay for the simultaneous quantification of morphine and its active metabolites morphine 3-glucuronide (M3G) and morphine 6-glucuronide (M6G) in human plasma and in dried blood spots (DBS). Reconstitution in water (DBS only) and addition of a protein precipitation solution containing the internal standards were the only manual steps. Morphine and its metabolites were separated on a Kinetex 2. 6-m PFP analytical column using an acetonitrile0. 1 formic acid gradient.

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